Team "Clinical Investigation Centre" (Nicolas Collongues)
Team "Translational Research" (Nicolas Collongues/Jérôme de Sèze)
email: nicolas.collongues[at]chru-strasbourg.fr & j.deseze[at]unistra.fr
Centre (CIC) of the Strasbourg University Hospitals coordinates numerous clinical research trials each year. In the field of pain, it facilitates clinical studies in chronic pain carried out by the Pain Evaluation and Treatment Centre (CETD, directed by Dr. Eric Salvat) but also those of other medical specialities such as the anesthesia-resuscitation (pain and COVID-19, postoperative pain), psychiatry-addictology (chronic pain and addiction, pain and mental health), pediatrics (pain of premature newborns), oncology, support and palliative care, etc. CIC 1434 also conducts translational biomedical research aimed at developing effective therapies against neurological and neurodegenerative diseases such as myelin pathologies (multiple sclerosis and neuromyelitis optica), peripheral neuropathies causing sensorimotor disorders and neuropathic pain. Thus CIC1434 gathers together basic researchers, clinical neurologists, engineers and technicians combining their complementary expertise to elucidate the mechanisms involved in these disorders and to characterize novel neuroprotective, anti-inflammatory and/or analgesic compounds to innovate safe and effective treatments of patients. The CIC is therefore an essential reception platform for improving pain treatments and practices in this area.
Techniques:
In vivo behavioral analysis platform (coupled with videotracking system) for animal models studies of :
Nociception: Plantar test ; von Frey filament test ; Acetone test
Motricity, Locomotion: Openfield ; Rotarod ; Grip test ; Catwalk system ; Running Wheel Activity test
Attention, Memory: Y-maze ; Morris Water Maze ; Object Recognition ; Barnes test ; Pattern Separation test ; 5-Choice Serial Reaction Time Test
Anxiety, Depression: Elevated Plus-maze ; Light/Dark ; PorsoltIn vitro analyses:
Cell Culture: Primary and line cultures ; Particularly neurons and oligodendrocytes ; MTT test ; Transfection ; Flow Cytometry, etc.
Protein Biochemistry: Western blot ; Chromatography ; Purification and enzymatic assays ; MSD biochemoluminescence dosages ; etc.
Molecular Biology: Nucleic acids extraction ; Electrophoresis ; Reverse transcription ; Semi-quantitative PCR ; Transgenesis ; RNASeq ; etc.
Subcellular particles isolation ; Mitochondria ; Synaptosomes ; etc.
Immunocyto- and -histochemistry: Cryostat ; Vibratome ; Fluorescent microscopy ; etc.